Affinity Chromatography: Methods and Protocols

Affinity Chromatography: Methods and Protocols (Methods in Molecular Biology

Product Details
Book Publisher: Humana Press (April, 2000)
ISBN: 0896036944
Book author: George K. Ehrlich, Wen-Jian Fung, Wolfgang Berthold, Pascal Bailon
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Book Description:
Hoffmann-La Roche Inc., Nutley, NJ. Describes methods in detail, ranging from traditional affinity purification methods as immunoaffinity chromatography, to the use phage-display technology in the discovery of affinity ligands and drugs. For researchers.

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RNA – Ligand Interactions, Part A

RNA – Ligand Interactions, Part A (Methods in Enzymology, Volume 317) (Methods in Enzymology)

Product Details
Book Publisher: Academic Press (May, 2000)
ISBN: 0121822184
Book author: John N. Abelson, Melvin I. Simon, Daniel W. Celander
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Book Description:
RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tertiary structure: Part B, its companion Volume 318 of Methods in Enzymology, focuses on molecular biology methods.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the Series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.

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Antifungal Agents

Antifungal Agents (Methods in Molecular Medicine)

Product Details
Book Publisher: Humana Press (16 May, 2005)
ISBN: 1588292770
Book author: Erika J. Ernst
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Book Description:
A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists and molecular biologists the productive tools they need today to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.

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Immunosuppressant Analogs in Neuroprotection

Immunosuppressant Analogs in Neuroprotection

Product Details
Book Publisher: Humana Press (20 November, 2002)
ISBN: 0896039447
Book author: Cesario V. Borlongan, Ole Isacson, Paul R. Sanberg
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Book Description:
An authoritative survey of the most recent scientific evidence showing how cyclosporin, FK-506, and their analogs-the neuroimmunophilins-evolved from being purely immunosuppressant drugs to neuroprotective agents. The book focuses on recent preclinical evidence that demonstrates the neurotrophic/neuroprotective effects of immunosuppressants when administered alone or when combined with neural transplantation therapy in animal models of neurological disorders. It also discusses their efficacy and mechanisms of action in vitro and in vivo models of CNS disease, and provides laboratory studies with compelling clinical indications for Alzheimers disease, Huntingtons disease, stroke, traumatic brain injury, spinal cord injury, ALS, sciatic nerve injury, and drug addiction

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Practical Preimplantation Genetic Diagnosis

Practical Preimplantation Genetic Diagnosis

Product Details
Book Publisher: Springer (21 October, 2005)
ISBN: 1852339209
Book author: Yury Verlinsky, Anver Kuliev
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Book Description:
Although treatment is the major goal in the control of genetic disease, this is not yet a reality for most of inherited conditions. Even with the dramatic advancement in field of gene therapy, there are unfortunately not enough success stories to allow us to predict its impact in the near future. Preimplantation genetic diagnosis (PGD) offers the answer to the control of these inherited conditions by predicting reproductive outcome. PGD is no longer an experimental procedure, having become an integral part of genetic practices and assisted reproduction technology. Therefore this practical book will be vital for clinicians to access a comprehensive description of available experience, indications, methods and clinical outcomes. Practical Preimplantation Genetic Diagnosis describes the experience of PGD, demonstrating the considerable progress made in this revolutionary procedure and describing its utility within different patient groups, the accuracy of the laboratory methods and prediction of the reproductive outcome. This will facilitate the use of PGD by practicing physicians, who may be able to offer patients this novel option for avoiding the birth of affected child and have a healthy children of their own. With the greatly improved accuracy of genetic analysis and indications expanding well beyond those for prenatal diagnosis, more than one thousand PGD cycles are now performed annually. PGD offers special features not available with traditional prenatal diagnosis. One is to avoid clinical pregnancy termination, which is especially attractive for couples carrying translocations, couples at risk for producing offspring with common diseases of autosomal dominant or recessive etiology, and for couples wishing to have not only an unaffected child, but an HLA compatible stem cell donor for treatment of an older moribund sib with a congenital disorder. Yet the greatest numerical impact of PGD is in assisted reproduction practices, where improved IVF efficiency through aneuploidy screening is evolving to become standard policy.

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Current Protocols in Neuroscience

Current Protocols in Neuroscience

Current Protocols In Neuroscience Guides You Step-By-Step Through Methods Used In Neuroscience Research.
Published by John Wiley & Sons, Current Protocols in Neuroscience meets the diverse needs of neuroscience researchers in the disciplines of neuroanatomy, cell biology, developmental biology, pharmacology, and molecular biology, in addition to the classical areas of behavioral science and neurophysiology.
Like all Current Protocols publications, Current Protocols in Neuroscience features carefully edited techniques with authors’ troubleshooting tips and helpful comments that come from extensive experience in using these procedures. Quarterly updates will keep you and your laboratory current with the latest developments in this rapidly changing field.
Format: Loose-leaf, 1-Year Subscription, Two Volumes
Item #: 978-0-471-16359-7
Price: $645.00 (US) Includes Shipping
January 1, 2008 – $700
Edited by: Jacqueline Crawley, Charles Gerfen, Michael Rogawski, David Sibley, Phil Skolnick, Susan Wray, Ron McKay (Past Editor) Series Editor: Gwen Taylor,
Short Protocols in Neuroscience: Cellular and Molecular Methods
Item #:978-0-471-78399-4
Format: Paperback, 592 pages, August 2006
Price: US $129.00
Short Protocols in Neuroscience: Systems and Behavioral Method
Jacqueline N. Crawley
Item #: 978-0-471-78397-8
Format: Paperback 610 pages, October 2006
Price: US $129.00

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Parasite Genomics Protocols

Parasite Genomics Protocols

By

Publisher: Humana Press
Number Of Pages: 472
Publication Date: 2004-05-17
Sales Rank: 877324
ISBN / ASIN: 158829062X
EAN: 9781588290625
Binding: Hardcover
Manufacturer: Humana Press
Book Description:

Pioneering bench researchers describe in detail the cutting-edge techniques they have developed for analyzing the genomes and gene products in a diverse range of protozoan and metazoan parasites. These readily reproducible techniques can be used in genomic, functional genomic, and post-genomic studies and include transfection methods and vectors for several protozoan parasites, global analysis using microarrays, gene ablation using RNA interference, gene knockout, mutagenesis, and chromosome manipulation. Some of the protocols require DNA sequence data, whereas others were developed independently of whole-genome sequence data. The protocols presented follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.

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Population Genetics: A Concise Guide

Population Genetics: A Concise Guide
By John H. Gillespie

• Publisher: The Johns Hopkins University Press
• Number Of Pages: 192
• Publication Date: 1997-09-05
• ISBN-10 / ASIN: 0801857554
• ISBN-13 / EAN: 9780801857553
• Binding: Paperback

Book Description:

“In a species with a million individuals,” writes John H. Gillespie, “it takes roughly a million generations for genetic drift to change allele frequencies appreciably. There is no conceivable way of verifying that genetic drift changes allele frequencies in most natural populations. Our understanding that it does is entirely theoretical. Most population geneticists are not only comfortable with this state of affairs, but revel in the fact that they can demonstrate on the back of an envelope, rather than in the laboratory, how an important evolutionary force operates.”
Longer than the back of an envelope but more concise than many books on the subject, this brief introduction to the field of population genetics offers students and researchers an overview of a discipline that is of growing importance. Chapter topics include genetic drift; natural selection; non-random mating, quantitative genetics; and the evolutionary advantage of . While each chapter treats a specific topic or problem in genetics, the common thread throughout the book is what Gillespie calls “the main obsession of our field,” the recurring question, “Why is there so much genetic variation in natural populations?”
“Population genetics remains the central intellectual connection between genetics and evolution. As genetics becomes integral to all aspects of biology, the unifying nature of evolutionary studies rests more and more on population genetics. This book lays out much of the foundation of population genetics augmented with interesting particulars and conceptual insight. Population genetics involves ideas that are quantitative and often difficult for biology undergraduates, but Professor Gillespie offers his characteristically clear thinking and articulate explanations.” — Charles Langley, University of California-Davis

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Histopathology Specimens: Clinical, Pathological and Laboratory Aspects

Histopathology Specimens: Clinical, Pathological and Laboratory Aspects
by

Histopathology Specimens: Clinical, Pathological and Laboratory Aspects
By Derek C. Allen, R. Iain Cameron

• Publisher: Springer
  Number Of Pages: 519
  Publication Date: 2004-01-31
  ISBN-10 / ASIN: 1852335971
  ISBN-13 / EAN: 9781852335977
• Binding: Paperback

Book Description:

Covering anatomical, clinical, pathological and laboratory aspects of surgical histopathology specimens, this book relates specimen dissection and its clinical context to relevant histopathology reports, and therefore a more comprehensive patient prognosis and management is possible.
Histopathology Specimens – Clinical, Pathological and Laboratory Aspects explains pathological and clinical terminology, including a glossary of clinical request form abbreviations. It offers a standardised step-wise approach to specimen handling illustrated by simple line diagrams and highlights essentials of the histopathology report, relating them to appropriate specimen dissection.
This book will act as a reference tool for the medical trainee in histopathology and the biomedical scientist, and as a useful aide memoire for the histopathology consultant.

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Laboratory Design, Construction, and Renovation: Participants, Process, and Product

Laboratory Design, Construction, and Renovation: Participants, Process, and Product

Laboratory facilities are complex, technically sophisticated, and mechanically intensive structures that are expensive to build and to maintain. Hundreds of decisions must be made before and during new construction or renovation that will determine how successfully the facility will function when completed and how successfully it can be maintained once put into service.

This book provides guidance on effective approaches for building laboratory facilities in the chemical and biochemical sciences. It contains both basic and laboratory-specific information addressed to the user community-the scientists and administrators who contract with design and construction experts. The book will also be important to the design and construction communities-the architects, laboratory designers, and engineers who will design the facility and the construction personnel who will build it-to help them communicate with the scientific community for whom they build laboratory facilities.

Executive Summary 1
1 Human Issues 8
Participants 8
Sociology 14
Community Relations 21
2 Process Issues 28
Predesign Phase 30
Preliminary Considerations 33
Design and Documentation Phase 38
Construction Phase 46
Postconstruction Phase 53
3 Technical Issues 59
Environmental Health and Safety 59
Design Considerations 72
Research Laboratory Cost Considerations 103
Bibliography 125
App. A Biographical Sketches of Committee Members 129
App. B Statement of Task 133
App. C Committee Meetings 134
App. D Selection of a Design Professional 137
App. E Definitions 143
Index 149

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